Construct: shRNA TRCN0000059365

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004996.2-1675s1c1
DNA Barcode:
CCACATGAAGAGCAAAGACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCC1 (4363)
Gene Description:
ATP binding cassette subfamily C member 1
Transcript:
RefSeq NM_004996.2 (NON-CURRENT)
Match location:
Position 1675 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059365)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACATGAAGAGCAAAGACAA
Hairpin Sequence:
5'-CCGG-CCACATGAAGAGCAAAGACAA-CTCGAG-TTGTCTTTGCTCTTCATGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACATGAAGAGCAAAGACAACTCGAGTTGTCTTTGCTCTTCATGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACATGAAGAGCAAAGACAACTCGAGTTGTCTTTGCTCTTCATGTGG-3'

Other clones with same target sequence:

  1. TRCN0000286611