Construct: shRNA TRCN0000059463

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005819.3-504s1c1
DNA Barcode:
GCAGGCATTAGCTGAAAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
STX6 (10228)
Gene Description:
syntaxin 6
Transcript:
RefSeq NM_005819.3 (NON-CURRENT)
Match location:
Position 504 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059463)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGCATTAGCTGAAAGAAA
Hairpin Sequence:
5'-CCGG-GCAGGCATTAGCTGAAAGAAA-CTCGAG-TTTCTTTCAGCTAATGCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGCATTAGCTGAAAGAAACTCGAGTTTCTTTCAGCTAATGCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGCATTAGCTGAAAGAAACTCGAGTTTCTTTCAGCTAATGCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000291917