Construct: shRNA TRCN0000059617

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178814.2-319s1c1
DNA Barcode:
GACAATGAGCTCTTGACGCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP1S3 (130340)
Gene Description:
adaptor related protein complex 1 subunit sigma 3
Transcript:
RefSeq NM_178814.2 (NON-CURRENT)
Match location:
Position 319 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059617)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACAATGAGCTCTTGACGCTA
Hairpin Sequence:
5'-CCGG-GACAATGAGCTCTTGACGCTA-CTCGAG-TAGCGTCAAGAGCTCATTGTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACAATGAGCTCTTGACGCTACTCGAGTAGCGTCAAGAGCTCATTGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACAATGAGCTCTTGACGCTACTCGAGTAGCGTCAAGAGCTCATTGTC-3'

Other clones with same target sequence:

(none)