Construct: shRNA TRCN0000059664

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006405.3-1496s1c1
DNA Barcode:
GCAGTACACTTTGTACGGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TM9SF1 (10548)
Gene Description:
transmembrane 9 superfamily member 1
Transcript:
RefSeq NM_006405.3 (NON-CURRENT)
Match location:
Position 1496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059664)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTACACTTTGTACGGCAT
Hairpin Sequence:
5'-CCGG-GCAGTACACTTTGTACGGCAT-CTCGAG-ATGCCGTACAAAGTGTACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTACACTTTGTACGGCATCTCGAGATGCCGTACAAAGTGTACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTACACTTTGTACGGCATCTCGAGATGCCGTACAAAGTGTACTGC-3'

Other clones with same target sequence:

  1. TRCN0000301129