Construct: shRNA TRCN0000059665

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006405.3-169s1c1
DNA Barcode:
CCTACCATAACCCTCAGGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TM9SF1 (10548)
Gene Description:
transmembrane 9 superfamily member 1
Transcript:
RefSeq NM_006405.3 (NON-CURRENT)
Match location:
Position 169 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059665)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCATAACCCTCAGGAAA
Hairpin Sequence:
5'-CCGG-CCTACCATAACCCTCAGGAAA-CTCGAG-TTTCCTGAGGGTTATGGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCATAACCCTCAGGAAACTCGAGTTTCCTGAGGGTTATGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCATAACCCTCAGGAAACTCGAGTTTCCTGAGGGTTATGGTAGG-3'

Other clones with same target sequence:

(none)