Construct: shRNA TRCN0000059683

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000927.3-3500s1c1
DNA Barcode:
CCGAACACATTGGAAGGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCB1 (5243)
Gene Description:
ATP binding cassette subfamily B member 1
Transcript:
RefSeq NM_000927.3 (NON-CURRENT)
Match location:
Position 3500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059683)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAACACATTGGAAGGAAAT
Hairpin Sequence:
5'-CCGG-CCGAACACATTGGAAGGAAAT-CTCGAG-ATTTCCTTCCAATGTGTTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAACACATTGGAAGGAAATCTCGAGATTTCCTTCCAATGTGTTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAACACATTGGAAGGAAATCTCGAGATTTCCTTCCAATGTGTTCGG-3'

Other clones with same target sequence:

(none)