Construct: shRNA TRCN0000059764

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006555.3-220s1c1
DNA Barcode:
CGCATACGATGTGTCTTCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YKT6 (10652)
Gene Description:
YKT6 v-SNARE homolog
Transcript:
RefSeq NM_006555.3 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059764)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATACGATGTGTCTTCCTT
Hairpin Sequence:
5'-CCGG-CGCATACGATGTGTCTTCCTT-CTCGAG-AAGGAAGACACATCGTATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATACGATGTGTCTTCCTTCTCGAGAAGGAAGACACATCGTATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATACGATGTGTCTTCCTTCTCGAGAAGGAAGACACATCGTATGCG-3'

Other clones with same target sequence:

  1. TRCN0000299710