Construct: shRNA TRCN0000059844

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007077.2-400s1c1
DNA Barcode:
CGAGATGGCTATTTATGAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP4S1 (11154)
Gene Description:
adaptor related protein complex 4 subunit sigma 1
Transcript:
RefSeq NM_007077.2 (NON-CURRENT)
Match location:
Position 400 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059844)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGAGATGGCTATTTATGAATT
Hairpin Sequence:
5'-CCGG-CGAGATGGCTATTTATGAATT-CTCGAG-AATTCATAAATAGCCATCTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGAGATGGCTATTTATGAATTCTCGAGAATTCATAAATAGCCATCTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGAGATGGCTATTTATGAATTCTCGAGAATTCATAAATAGCCATCTCG-3'

Other clones with same target sequence:

(none)