Construct: shRNA TRCN0000059850

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002858.2-985s1c1
DNA Barcode:
GCCAAATACCTTGCCACTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCD3 (5825)
Gene Description:
ATP binding cassette subfamily D member 3
Transcript:
RefSeq NM_002858.2 (NON-CURRENT)
Match location:
Position 985 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059850)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCAAATACCTTGCCACTGTT
Hairpin Sequence:
5'-CCGG-GCCAAATACCTTGCCACTGTT-CTCGAG-AACAGTGGCAAGGTATTTGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCAAATACCTTGCCACTGTTCTCGAGAACAGTGGCAAGGTATTTGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCAAATACCTTGCCACTGTTCTCGAGAACAGTGGCAAGGTATTTGGC-3'

Other clones with same target sequence:

(none)