Construct: shRNA TRCN0000059932

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031912.3-1084s1c1
DNA Barcode:
AGAGGCATTGTCAGTGTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYT15 (83849)
Gene Description:
synaptotagmin 15
Transcript:
RefSeq NM_031912.3 (NON-CURRENT)
Match location:
Position 1084 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059932)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGCATTGTCAGTGTGTTT
Hairpin Sequence:
5'-CCGG-AGAGGCATTGTCAGTGTGTTT-CTCGAG-AAACACACTGACAATGCCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGCATTGTCAGTGTGTTTCTCGAGAAACACACTGACAATGCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGCATTGTCAGTGTGTTTCTCGAGAAACACACTGACAATGCCTCT-3'

Other clones with same target sequence:

(none)