Construct: shRNA TRCN0000059948

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032379.3-1472s1c1
DNA Barcode:
CGGTGGAATCTGATCTTGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYTL2 (54843)
Gene Description:
synaptotagmin like 2
Transcript:
RefSeq NM_032379.3 (NON-CURRENT)
Match location:
Position 1472 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059948)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGGAATCTGATCTTGAAA
Hairpin Sequence:
5'-CCGG-CGGTGGAATCTGATCTTGAAA-CTCGAG-TTTCAAGATCAGATTCCACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGGAATCTGATCTTGAAACTCGAGTTTCAAGATCAGATTCCACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGGAATCTGATCTTGAAACTCGAGTTTCAAGATCAGATTCCACCG-3'

Other clones with same target sequence:

(none)