Construct: shRNA TRCN0000059952

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032379.3-1146s1c1
DNA Barcode:
CCCTATCTTCAACCACACTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYTL2 (54843)
Gene Description:
synaptotagmin like 2
Transcript:
RefSeq NM_032379.3 (NON-CURRENT)
Match location:
Position 1146 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000059952)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTATCTTCAACCACACTAT
Hairpin Sequence:
5'-CCGG-CCCTATCTTCAACCACACTAT-CTCGAG-ATAGTGTGGTTGAAGATAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTATCTTCAACCACACTATCTCGAGATAGTGTGGTTGAAGATAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTATCTTCAACCACACTATCTCGAGATAGTGTGGTTGAAGATAGGG-3'

Other clones with same target sequence:

(none)