Construct: shRNA TRCN0000060055

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174975.2-885s1c1
DNA Barcode:
GTGGAATACGAGATCCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEC14L3 (266629)
Gene Description:
SEC14 like lipid binding 3
Transcript:
RefSeq NM_174975.2 (NON-CURRENT)
Match location:
Position 885 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060055)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGGAATACGAGATCCTATTT
Hairpin Sequence:
5'-CCGG-GTGGAATACGAGATCCTATTT-CTCGAG-AAATAGGATCTCGTATTCCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGGAATACGAGATCCTATTTCTCGAGAAATAGGATCTCGTATTCCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGGAATACGAGATCCTATTTCTCGAGAAATAGGATCTCGTATTCCAC-3'

Other clones with same target sequence:

(none)