Construct: shRNA TRCN0000060069

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172020.1-1477s1c1
DNA Barcode:
CCAGTTCATCACCCTTCTCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POM121 (9883)
Gene Description:
POM121 transmembrane nucleoporin
Transcript:
RefSeq NM_172020.1 (NON-CURRENT)
Match location:
Position 1477 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060069)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTTCATCACCCTTCTCTA
Hairpin Sequence:
5'-CCGG-CCAGTTCATCACCCTTCTCTA-CTCGAG-TAGAGAAGGGTGATGAACTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTTCATCACCCTTCTCTACTCGAGTAGAGAAGGGTGATGAACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTTCATCACCCTTCTCTACTCGAGTAGAGAAGGGTGATGAACTGG-3'

Other clones with same target sequence:

(none)