Construct: shRNA TRCN0000060150
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_003003.1-520s1c1
- DNA Barcode:
- GCAGGAGTTGATTATGTTTAT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- SEC14L1 (6397)
- Gene Description:
- SEC14 like lipid binding 1
- Transcript:
- RefSeq NM_003003.1 (NON-CURRENT)
- Match location:
- Position 520 (CDS)
Vector Information:
- Vector Backbone:
- pLKO.1
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000060150)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCAGGAGTTGATTATGTTTAT
- Hairpin Sequence:
-
5'-CCGG-GCAGGAGTTGATTATGTTTAT-CTCGAG-ATAAACATAATCAACTCCTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCAGGAGTTGATTATGTTTATCTCGAGATAAACATAATCAACTCCTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCAGGAGTTGATTATGTTTATCTCGAGATAAACATAATCAACTCCTGC-3'
Other clones with same target sequence:
-
TRCN0000299467
