Construct: shRNA TRCN0000060225

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004504.3-1696s1c1
DNA Barcode:
GCTGCTGGAGTATCTAGTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AGFG1 (3267)
Gene Description:
ArfGAP with FG repeats 1
Transcript:
RefSeq NM_004504.3 (NON-CURRENT)
Match location:
Position 1696 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060225)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGGAGTATCTAGTAAT
Hairpin Sequence:
5'-CCGG-GCTGCTGGAGTATCTAGTAAT-CTCGAG-ATTACTAGATACTCCAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGGAGTATCTAGTAATCTCGAGATTACTAGATACTCCAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGGAGTATCTAGTAATCTCGAGATTACTAGATACTCCAGCAGC-3'

Other clones with same target sequence:

(none)