Construct: shRNA TRCN0000060243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005124.2-2469s1c1
DNA Barcode:
GCCCTTACATTGACAGTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUP153 (9972)
Gene Description:
nucleoporin 153
Transcript:
RefSeq NM_005124.2 (NON-CURRENT)
Match location:
Position 2469 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTACATTGACAGTGGTT
Hairpin Sequence:
5'-CCGG-GCCCTTACATTGACAGTGGTT-CTCGAG-AACCACTGTCAATGTAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTACATTGACAGTGGTTCTCGAGAACCACTGTCAATGTAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTACATTGACAGTGGTTCTCGAGAACCACTGTCAATGTAAGGGC-3'

Other clones with same target sequence:

(none)