Construct: shRNA TRCN0000060300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144579.1-574s1c1
DNA Barcode:
GTCAACTATGCAAACCGCAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SFXN5 (94097)
Gene Description:
sideroflexin 5
Transcript:
RefSeq NM_144579.1 (NON-CURRENT)
Match location:
Position 574 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAACTATGCAAACCGCAAT
Hairpin Sequence:
5'-CCGG-GTCAACTATGCAAACCGCAAT-CTCGAG-ATTGCGGTTTGCATAGTTGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAACTATGCAAACCGCAATCTCGAGATTGCGGTTTGCATAGTTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAACTATGCAAACCGCAATCTCGAGATTGCGGTTTGCATAGTTGAC-3'

Other clones with same target sequence:

  1. TRCN0000413399