Construct: shRNA TRCN0000060315

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001284.1-418s1c1
DNA Barcode:
CCATGTAGACAAGGTTCACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP3S1 (1176)
Gene Description:
adaptor related protein complex 3 subunit sigma 1
Transcript:
RefSeq NM_001284.1 (NON-CURRENT)
Match location:
Position 418 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060315)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTAGACAAGGTTCACAA
Hairpin Sequence:
5'-CCGG-CCATGTAGACAAGGTTCACAA-CTCGAG-TTGTGAACCTTGTCTACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTAGACAAGGTTCACAACTCGAGTTGTGAACCTTGTCTACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTAGACAAGGTTCACAACTCGAGTTGTGAACCTTGTCTACATGG-3'

Other clones with same target sequence:

  1. TRCN0000290102