Construct: shRNA TRCN0000060371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004460.1-134s1c1
DNA Barcode:
CCATGTACTTCTTCCTCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR10A2 (341276)
Gene Description:
olfactory receptor family 10 subfamily A member 2
Transcript:
RefSeq NM_001004460.1 (CURRENT)
Match location:
Position 134 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATGTACTTCTTCCTCAGAA
Hairpin Sequence:
5'-CCGG-CCATGTACTTCTTCCTCAGAA-CTCGAG-TTCTGAGGAAGAAGTACATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATGTACTTCTTCCTCAGAACTCGAGTTCTGAGGAAGAAGTACATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATGTACTTCTTCCTCAGAACTCGAGTTCTGAGGAAGAAGTACATGG-3'

Other clones with same target sequence:

(none)