Construct: shRNA TRCN0000060628

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005264.2-1785s1c1
DNA Barcode:
CCTCTGTATTTCCAATGGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFRA1 (2674)
Gene Description:
GDNF family receptor alpha 1
Transcript:
RefSeq NM_005264.2 (NON-CURRENT)
Match location:
Position 1785 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060628)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTGTATTTCCAATGGTAA
Hairpin Sequence:
5'-CCGG-CCTCTGTATTTCCAATGGTAA-CTCGAG-TTACCATTGGAAATACAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTGTATTTCCAATGGTAACTCGAGTTACCATTGGAAATACAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTGTATTTCCAATGGTAACTCGAGTTACCATTGGAAATACAGAGG-3'

Other clones with same target sequence:

(none)