Construct: shRNA TRCN0000060678

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198153.1-403s1c1
DNA Barcode:
TGACACAGAATGACTCGGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TREML4 (285852)
Gene Description:
triggering receptor expressed on myeloid cells like 4
Transcript:
RefSeq NM_198153.1 (NON-CURRENT)
Match location:
Position 403 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060678)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACACAGAATGACTCGGGAT
Hairpin Sequence:
5'-CCGG-TGACACAGAATGACTCGGGAT-CTCGAG-ATCCCGAGTCATTCTGTGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACACAGAATGACTCGGGATCTCGAGATCCCGAGTCATTCTGTGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACACAGAATGACTCGGGATCTCGAGATCCCGAGTCATTCTGTGTCA-3'

Other clones with same target sequence:

(none)