Construct: shRNA TRCN0000060739

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001496.3-516s1c1
DNA Barcode:
CTTGGTAACTATGAGCTGGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFRA3 (2676)
Gene Description:
GDNF family receptor alpha 3
Transcript:
RefSeq NM_001496.3 (NON-CURRENT)
Match location:
Position 516 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060739)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGGTAACTATGAGCTGGAT
Hairpin Sequence:
5'-CCGG-CTTGGTAACTATGAGCTGGAT-CTCGAG-ATCCAGCTCATAGTTACCAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGGTAACTATGAGCTGGATCTCGAGATCCAGCTCATAGTTACCAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGGTAACTATGAGCTGGATCTCGAGATCCAGCTCATAGTTACCAAG-3'

Other clones with same target sequence:

(none)