Construct: shRNA TRCN0000060759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018708.1-556s1c1
DNA Barcode:
CCTCATGATCTCGTGCTACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FEM1A (55527)
Gene Description:
fem-1 homolog A
Transcript:
RefSeq NM_018708.1 (NON-CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCATGATCTCGTGCTACAA
Hairpin Sequence:
5'-CCGG-CCTCATGATCTCGTGCTACAA-CTCGAG-TTGTAGCACGAGATCATGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCATGATCTCGTGCTACAACTCGAGTTGTAGCACGAGATCATGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCATGATCTCGTGCTACAACTCGAGTTGTAGCACGAGATCATGAGG-3'

Other clones with same target sequence:

(none)