Construct: shRNA TRCN0000060845

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024897.2-1288s1c1
DNA Barcode:
GCTGGGAACCTACTCATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PAQR6 (79957)
Gene Description:
progestin and adipoQ receptor family member 6
Transcript:
RefSeq NM_024897.2 (NON-CURRENT)
Match location:
Position 1288 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060845)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGGAACCTACTCATTATT
Hairpin Sequence:
5'-CCGG-GCTGGGAACCTACTCATTATT-CTCGAG-AATAATGAGTAGGTTCCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGGAACCTACTCATTATTCTCGAGAATAATGAGTAGGTTCCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGGAACCTACTCATTATTCTCGAGAATAATGAGTAGGTTCCCAGC-3'

Other clones with same target sequence:

(none)