Construct: shRNA TRCN0000060950

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006340.1-1341s1c1
DNA Barcode:
AGAGAGTGAGAAGACCAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BAIAP2 (10458)
Gene Description:
BAR/IMD domain containing adaptor protein 2
Transcript:
RefSeq NM_006340.1 (NON-CURRENT)
Match location:
Position 1341 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060950)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGAGTGAGAAGACCAAGAT
Hairpin Sequence:
5'-CCGG-AGAGAGTGAGAAGACCAAGAT-CTCGAG-ATCTTGGTCTTCTCACTCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGAGTGAGAAGACCAAGATCTCGAGATCTTGGTCTTCTCACTCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGAGTGAGAAGACCAAGATCTCGAGATCTTGGTCTTCTCACTCTCT-3'

Other clones with same target sequence:

  1. TRCN0000291326