Construct: shRNA TRCN0000060995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013440.3-2681s1c1
DNA Barcode:
CCATAGTTCCCAACGTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PILRB (29990)
Gene Description:
paired immunoglobin like type 2 receptor beta
Transcript:
RefSeq NM_013440.3 (NON-CURRENT)
Match location:
Position 2681 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000060995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATAGTTCCCAACGTGAGAA
Hairpin Sequence:
5'-CCGG-CCATAGTTCCCAACGTGAGAA-CTCGAG-TTCTCACGTTGGGAACTATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATAGTTCCCAACGTGAGAACTCGAGTTCTCACGTTGGGAACTATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATAGTTCCCAACGTGAGAACTCGAGTTCTCACGTTGGGAACTATGG-3'

Other clones with same target sequence:

  1. TRCN0000291329