Construct: shRNA TRCN0000061106

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000744.2-1814s1c1
DNA Barcode:
CGTGCAAATGCACATGCAAGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRNA4 (1137)
Gene Description:
cholinergic receptor nicotinic alpha 4 subunit
Transcript:
RefSeq NM_000744.2 (NON-CURRENT)
Match location:
Position 1814 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061106)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGCAAATGCACATGCAAGA
Hairpin Sequence:
5'-CCGG-CGTGCAAATGCACATGCAAGA-CTCGAG-TCTTGCATGTGCATTTGCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGCAAATGCACATGCAAGACTCGAGTCTTGCATGTGCATTTGCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGCAAATGCACATGCAAGACTCGAGTCTTGCATGTGCATTTGCACG-3'

Other clones with same target sequence:

(none)