Construct: shRNA TRCN0000061109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020796.1-1890s1c1
DNA Barcode:
CCTGAGAACAATGGTCAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA6A (57556)
Gene Description:
semaphorin 6A
Transcript:
RefSeq NM_020796.1 (NON-CURRENT)
Match location:
Position 1890 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAGAACAATGGTCAGATA
Hairpin Sequence:
5'-CCGG-CCTGAGAACAATGGTCAGATA-CTCGAG-TATCTGACCATTGTTCTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAGAACAATGGTCAGATACTCGAGTATCTGACCATTGTTCTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAGAACAATGGTCAGATACTCGAGTATCTGACCATTGTTCTCAGG-3'

Other clones with same target sequence:

(none)