Construct: shRNA TRCN0000061121

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012428.1-738s1c1
DNA Barcode:
CATGGAGTACAGGATCAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPTN (27020)
Gene Description:
neuroplastin
Transcript:
RefSeq NM_012428.1 (NON-CURRENT)
Match location:
Position 738 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061121)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGGAGTACAGGATCAATAA
Hairpin Sequence:
5'-CCGG-CATGGAGTACAGGATCAATAA-CTCGAG-TTATTGATCCTGTACTCCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGGAGTACAGGATCAATAACTCGAGTTATTGATCCTGTACTCCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGGAGTACAGGATCAATAACTCGAGTTATTGATCCTGTACTCCATG-3'

Other clones with same target sequence:

  1. TRCN0000247865
  2. TRCN0000193073
  3. TRCN0000291393