Construct: shRNA TRCN0000061134

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000745.2-496s1c1
DNA Barcode:
CCTGATGACTATGGTGGAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRNA5 (1138)
Gene Description:
cholinergic receptor nicotinic alpha 5 subunit
Transcript:
RefSeq NM_000745.2 (NON-CURRENT)
Match location:
Position 496 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061134)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGATGACTATGGTGGAATA
Hairpin Sequence:
5'-CCGG-CCTGATGACTATGGTGGAATA-CTCGAG-TATTCCACCATAGTCATCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGATGACTATGGTGGAATACTCGAGTATTCCACCATAGTCATCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGATGACTATGGTGGAATACTCGAGTATTCCACCATAGTCATCAGG-3'

Other clones with same target sequence:

  1. TRCN0000291203