Construct: shRNA TRCN0000061140

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017893.2-1508s1c1
DNA Barcode:
GCTCGTGTATCACAGATTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEMA4G (57715)
Gene Description:
semaphorin 4G
Transcript:
RefSeq NM_017893.2 (NON-CURRENT)
Match location:
Position 1508 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061140)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTCGTGTATCACAGATTCAT
Hairpin Sequence:
5'-CCGG-GCTCGTGTATCACAGATTCAT-CTCGAG-ATGAATCTGTGATACACGAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTCGTGTATCACAGATTCATCTCGAGATGAATCTGTGATACACGAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTCGTGTATCACAGATTCATCTCGAGATGAATCTGTGATACACGAGC-3'

Other clones with same target sequence:

  1. TRCN0000291198