Construct: shRNA TRCN0000061223

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000748.1-927s1c1
DNA Barcode:
CGTGGACATCACGTATGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRNB2 (1141)
Gene Description:
cholinergic receptor nicotinic beta 2 subunit
Transcript:
RefSeq NM_000748.1 (NON-CURRENT)
Match location:
Position 927 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061223)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGGACATCACGTATGACTT
Hairpin Sequence:
5'-CCGG-CGTGGACATCACGTATGACTT-CTCGAG-AAGTCATACGTGATGTCCACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGGACATCACGTATGACTTCTCGAGAAGTCATACGTGATGTCCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGGACATCACGTATGACTTCTCGAGAAGTCATACGTGATGTCCACG-3'

Other clones with same target sequence:

(none)