Construct: shRNA TRCN0000061247

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002222.1-671s1c1
DNA Barcode:
CCTGCTCTGTTGGAGAAGAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITPR1 (3708)
Gene Description:
inositol 1,4,5-trisphosphate receptor type 1
Transcript:
RefSeq NM_002222.1 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061247)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGCTCTGTTGGAGAAGAAT
Hairpin Sequence:
5'-CCGG-CCTGCTCTGTTGGAGAAGAAT-CTCGAG-ATTCTTCTCCAACAGAGCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGCTCTGTTGGAGAAGAATCTCGAGATTCTTCTCCAACAGAGCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGCTCTGTTGGAGAAGAATCTCGAGATTCTTCTCCAACAGAGCAGG-3'

Other clones with same target sequence:

(none)