Construct: shRNA TRCN0000061260

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000749.2-261s1c1
DNA Barcode:
CGCCCTGTATTACATTCTAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRNB3 (1142)
Gene Description:
cholinergic receptor nicotinic beta 3 subunit
Transcript:
RefSeq NM_000749.2 (NON-CURRENT)
Match location:
Position 261 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061260)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCCCTGTATTACATTCTAAT
Hairpin Sequence:
5'-CCGG-CGCCCTGTATTACATTCTAAT-CTCGAG-ATTAGAATGTAATACAGGGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCCCTGTATTACATTCTAATCTCGAGATTAGAATGTAATACAGGGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCCCTGTATTACATTCTAATCTCGAGATTAGAATGTAATACAGGGCG-3'

Other clones with same target sequence:

  1. TRCN0000300605