Construct: shRNA TRCN0000061268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000810.2-1650s1c1
DNA Barcode:
CGGCACTTTCAACTTAGTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRA5 (2558)
Gene Description:
gamma-aminobutyric acid type A receptor alpha5 subunit
Transcript:
RefSeq NM_000810.2 (NON-CURRENT)
Match location:
Position 1650 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGCACTTTCAACTTAGTTTA
Hairpin Sequence:
5'-CCGG-CGGCACTTTCAACTTAGTTTA-CTCGAG-TAAACTAAGTTGAAAGTGCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGCACTTTCAACTTAGTTTACTCGAGTAAACTAAGTTGAAAGTGCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGCACTTTCAACTTAGTTTACTCGAGTAAACTAAGTTGAAAGTGCCG-3'

Other clones with same target sequence:

(none)