Construct: shRNA TRCN0000061296

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004687.1-663s1c1
DNA Barcode:
CCTTGCTGTCTACCACATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR2L3 (391192)
Gene Description:
olfactory receptor family 2 subfamily L member 3
Transcript:
RefSeq NM_001004687.1 (CURRENT)
Match location:
Position 663 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061296)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTGTCTACCACATGAA
Hairpin Sequence:
5'-CCGG-CCTTGCTGTCTACCACATGAA-CTCGAG-TTCATGTGGTAGACAGCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTGTCTACCACATGAACTCGAGTTCATGTGGTAGACAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTGTCTACCACATGAACTCGAGTTCATGTGGTAGACAGCAAGG-3'

Other clones with same target sequence:

(none)