Construct: shRNA TRCN0000061323

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002224.1-6672s1c1
DNA Barcode:
CGTGTTTATCAACATCATCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITPR3 (3710)
Gene Description:
inositol 1,4,5-trisphosphate receptor type 3
Transcript:
RefSeq NM_002224.1 (NON-CURRENT)
Match location:
Position 6672 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061323)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGTTTATCAACATCATCAT
Hairpin Sequence:
5'-CCGG-CGTGTTTATCAACATCATCAT-CTCGAG-ATGATGATGTTGATAAACACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGTTTATCAACATCATCATCTCGAGATGATGATGTTGATAAACACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGTTTATCAACATCATCATCTCGAGATGATGATGTTGATAAACACG-3'

Other clones with same target sequence:

  1. TRCN0000333313