Construct: shRNA TRCN0000061394

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000813.1-1578s1c1
DNA Barcode:
CGCATATTCTTCCCAGTGGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GABRB2 (2561)
Gene Description:
gamma-aminobutyric acid type A receptor beta2 subunit
Transcript:
RefSeq NM_000813.1 (NON-CURRENT)
Match location:
Position 1578 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061394)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCATATTCTTCCCAGTGGTT
Hairpin Sequence:
5'-CCGG-CGCATATTCTTCCCAGTGGTT-CTCGAG-AACCACTGGGAAGAATATGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCATATTCTTCCCAGTGGTTCTCGAGAACCACTGGGAAGAATATGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCATATTCTTCCCAGTGGTTCTCGAGAACCACTGGGAAGAATATGCG-3'

Other clones with same target sequence:

(none)