Construct: shRNA TRCN0000061407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004457.1-821s1c1
DNA Barcode:
GTGTGTATTTACTTCCTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
OR1N2 (138882)
Gene Description:
olfactory receptor family 1 subfamily N member 2
Transcript:
RefSeq NM_001004457.1 (NON-CURRENT)
Match location:
Position 821 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGTGTATTTACTTCCTCCAT
Hairpin Sequence:
5'-CCGG-GTGTGTATTTACTTCCTCCAT-CTCGAG-ATGGAGGAAGTAAATACACAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGTGTATTTACTTCCTCCATCTCGAGATGGAGGAAGTAAATACACACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGTGTATTTACTTCCTCCATCTCGAGATGGAGGAAGTAAATACACAC-3'

Other clones with same target sequence:

(none)