Construct: shRNA TRCN0000061435

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022065.3-4933s1c1
DNA Barcode:
CCAGAATGCTTCTGCAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
THADA (63892)
Gene Description:
THADA armadillo repeat containing
Transcript:
RefSeq NM_022065.3 (NON-CURRENT)
Match location:
Position 4933 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061435)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGAATGCTTCTGCAAGATA
Hairpin Sequence:
5'-CCGG-CCAGAATGCTTCTGCAAGATA-CTCGAG-TATCTTGCAGAAGCATTCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGAATGCTTCTGCAAGATACTCGAGTATCTTGCAGAAGCATTCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGAATGCTTCTGCAAGATACTCGAGTATCTTGCAGAAGCATTCTGG-3'

Other clones with same target sequence:

(none)