Construct: shRNA TRCN0000061529

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015532.2-1057s1c1
DNA Barcode:
GCACTTCAGAAACAGCAGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLR2M (81488)
Gene Description:
RNA polymerase II subunit M
Transcript:
RefSeq NM_015532.2 (NON-CURRENT)
Match location:
Position 1057 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061529)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTTCAGAAACAGCAGAAA
Hairpin Sequence:
5'-CCGG-GCACTTCAGAAACAGCAGAAA-CTCGAG-TTTCTGCTGTTTCTGAAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTTCAGAAACAGCAGAAACTCGAGTTTCTGCTGTTTCTGAAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTTCAGAAACAGCAGAAACTCGAGTTTCTGCTGTTTCTGAAGTGC-3'

Other clones with same target sequence:

(none)