Construct: shRNA TRCN0000061585

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002255.3-209s1c1
DNA Barcode:
GTTTAACATCTTCACGCTGTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KIR2DL4 (3805)
Gene Description:
killer cell immunoglobulin like receptor, two Ig domains and long cytoplasmic tail 4
Transcript:
RefSeq NM_002255.3 (NON-CURRENT)
Match location:
Position 209 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061585)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTTAACATCTTCACGCTGTA
Hairpin Sequence:
5'-CCGG-GTTTAACATCTTCACGCTGTA-CTCGAG-TACAGCGTGAAGATGTTAAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTTAACATCTTCACGCTGTACTCGAGTACAGCGTGAAGATGTTAAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTTAACATCTTCACGCTGTACTCGAGTACAGCGTGAAGATGTTAAAC-3'

Other clones with same target sequence:

(none)