Construct: shRNA TRCN0000061599

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004490.1-1243s1c1
DNA Barcode:
CCTGAAATTCATGGTTTCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRB14 (2888)
Gene Description:
growth factor receptor bound protein 14
Transcript:
RefSeq NM_004490.1 (NON-CURRENT)
Match location:
Position 1243 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061599)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAATTCATGGTTTCTTA
Hairpin Sequence:
5'-CCGG-CCTGAAATTCATGGTTTCTTA-CTCGAG-TAAGAAACCATGAATTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAATTCATGGTTTCTTACTCGAGTAAGAAACCATGAATTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAATTCATGGTTTCTTACTCGAGTAAGAAACCATGAATTTCAGG-3'

Other clones with same target sequence:

  1. TRCN0000300082