Construct: shRNA TRCN0000061639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000827.2-314s1c1
DNA Barcode:
CCAGATTGATATTGTGAACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIA1 (2890)
Gene Description:
glutamate ionotropic receptor AMPA type subunit 1
Transcript:
RefSeq NM_000827.2 (NON-CURRENT)
Match location:
Position 314 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATTGATATTGTGAACAT
Hairpin Sequence:
5'-CCGG-CCAGATTGATATTGTGAACAT-CTCGAG-ATGTTCACAATATCAATCTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATTGATATTGTGAACATCTCGAGATGTTCACAATATCAATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATTGATATTGTGAACATCTCGAGATGTTCACAATATCAATCTGG-3'

Other clones with same target sequence:

(none)