Construct: shRNA TRCN0000061671

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002063.2-1743s1c1
DNA Barcode:
GATGCTATCAAGAAGAAGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GLRA2 (2742)
Gene Description:
glycine receptor alpha 2
Transcript:
RefSeq NM_002063.2 (NON-CURRENT)
Match location:
Position 1743 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061671)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGCTATCAAGAAGAAGTTT
Hairpin Sequence:
5'-CCGG-GATGCTATCAAGAAGAAGTTT-CTCGAG-AAACTTCTTCTTGATAGCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGCTATCAAGAAGAAGTTTCTCGAGAAACTTCTTCTTGATAGCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGCTATCAAGAAGAAGTTTCTCGAGAAACTTCTTCTTGATAGCATC-3'

Other clones with same target sequence:

(none)