Construct: shRNA TRCN0000061738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000021.2-1561s1c1
DNA Barcode:
CCATCACCTTTGGGCTTGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PSEN1 (5663)
Gene Description:
presenilin 1
Transcript:
RefSeq NM_000021.2 (NON-CURRENT)
Match location:
Position 1561 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCATCACCTTTGGGCTTGTTT
Hairpin Sequence:
5'-CCGG-CCATCACCTTTGGGCTTGTTT-CTCGAG-AAACAAGCCCAAAGGTGATGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCATCACCTTTGGGCTTGTTTCTCGAGAAACAAGCCCAAAGGTGATGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCATCACCTTTGGGCTTGTTTCTCGAGAAACAAGCCCAAAGGTGATGG-3'

Other clones with same target sequence:

  1. TRCN0000315761