Construct: shRNA TRCN0000061752

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005801.2-384s1c1
DNA Barcode:
TGGAGAAGTAATTCAGCTACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EIF1 (10209)
Gene Description:
eukaryotic translation initiation factor 1
Transcript:
RefSeq NM_005801.2 (NON-CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061752)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGAAGTAATTCAGCTACA
Hairpin Sequence:
5'-CCGG-TGGAGAAGTAATTCAGCTACA-CTCGAG-TGTAGCTGAATTACTTCTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGAAGTAATTCAGCTACACTCGAGTGTAGCTGAATTACTTCTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGAAGTAATTCAGCTACACTCGAGTGTAGCTGAATTACTTCTCCA-3'

Other clones with same target sequence:

  1. TRCN0000315700