Construct: shRNA TRCN0000061796

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005426.1-1282s1c1
DNA Barcode:
GCAGTCATGGATAAGCGTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TP53BP2 (7159)
Gene Description:
tumor protein p53 binding protein 2
Transcript:
RefSeq NM_005426.1 (NON-CURRENT)
Match location:
Position 1282 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061796)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTCATGGATAAGCGTGTT
Hairpin Sequence:
5'-CCGG-GCAGTCATGGATAAGCGTGTT-CTCGAG-AACACGCTTATCCATGACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTCATGGATAAGCGTGTTCTCGAGAACACGCTTATCCATGACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTCATGGATAAGCGTGTTCTCGAGAACACGCTTATCCATGACTGC-3'

Other clones with same target sequence:

  1. TRCN0000315702