Construct: shRNA TRCN0000061832

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014788.2-362s1c1
DNA Barcode:
CTCAGATTACTACTTGACGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRIM14 (9830)
Gene Description:
tripartite motif containing 14
Transcript:
RefSeq NM_014788.2 (NON-CURRENT)
Match location:
Position 362 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000061832)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAGATTACTACTTGACGAA
Hairpin Sequence:
5'-CCGG-CTCAGATTACTACTTGACGAA-CTCGAG-TTCGTCAAGTAGTAATCTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAGATTACTACTTGACGAACTCGAGTTCGTCAAGTAGTAATCTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAGATTACTACTTGACGAACTCGAGTTCGTCAAGTAGTAATCTGAG-3'

Other clones with same target sequence:

(none)